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RP105 antibody [N2C1], Internal

Anti-RP105 antibody [N2C1], Internal used in Western Blot (WB). GTX109057
Anti-RP105 antibody [N2C1], Internal used in Flow cytometry (FACS). GTX109057
Anti-RP105 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX109057

Cat. No. GTX109057

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all RP105 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

Jurkat

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human RP105. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10727211

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD180 molecule , LY64 , Ly78 , RP105

Cellular Localization

Cell membrane

Background

CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RP105 antibody [N2C1], Internal used in Western Blot (WB). GTX109057

GTX109057 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX109057 diluted at 1:1000

Anti-RP105 antibody [N2C1], Internal used in Flow cytometry (FACS). GTX109057

GTX109057 FACS Image

RP105 antibody [N2C1], Internal Flow cytometry on Jurkat cells fixed in 4% paraformaldehyde at 4oC for 5 min, staining with RP105 (GTX109057) antibody at 1:100 dilution (blue) or rabbit IgG (black).

Anti-RP105 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX109057

GTX109057 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using RP105(GTX109057) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Kikuchi J et al. Cancer Res 2018; (Epub) Myeloma Cells are Activated in Bone Marrow Microenvironment by the CD180/MD-1 Complex which Senses Lipopolysaccharide.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169