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RPA14 antibody [11.1]

Anti-RPA14 antibody [11.1] used in Western Blot (WB). GTX70238
Anti-RPA14 antibody [11.1] used in Western Blot (WB). GTX70238
Anti-RPA14 antibody [11.1] used in IHC (Paraffin sections) (IHC-P). GTX70238

Cat No. GTX70238

Host

Mouse

Clonality

Monoclonal

Clone Name

11.1

Isotype

IgG1

Application

WB, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1 - 2 μg/ml
IHC-P 1:100-1:1000
IP 0.1 - 2 μg/ml
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

MCF7, T24

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length human RPA-14 expressed in E. coli..

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Replication Protein A3 , Repa3 , Rp-A P14 , Rpa3

Cellular Localization

Nuclear

Background

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.

Database

Research Area

DATA IMAGES

Anti-RPA14 antibody [11.1] used in Western Blot (WB). GTX70238

GTX70238 WB Image

RPA14 antibody [11.1] detects RPA14 protein by Western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg MCF-7 whole cell lysate/extract
15 % SDS-PAGE
RPA14 antibody [11.1] (GTX70238) dilution: 1:1000

Anti-RPA14 antibody [11.1] used in Western Blot (WB). GTX70238

GTX70238 WB Image

Sample (30 ug of whole cell lysate)
A: MCF7
15% SDS PAGE
GTX70238 diluted at 1:500

Anti-RPA14 antibody [11.1] used in IHC (Paraffin sections) (IHC-P). GTX70238

GTX70238 IHC-P Image

RPA14 antibody [11.1] detects RPA14 protein at nucleus on Mahlarvu xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Mahlarvu xenograft.
RPA14 antibody [11.1] (GTX70238) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329