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RPA14 antibody [14.2]

Anti-RPA14 antibody [14.2] used in Western Blot (WB). GTX70240

Cat No. GTX70240

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 14.2

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($329)
See all RPA14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1 - 2 μg/ml
IP 0.1 - 2 μg/ml
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

MCF7, T24

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length human RPA-14 expressed in E. coli..

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Replication Protein A3 , Repa3 , Rp-A P14 , Rpa3

Background

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.

Database

Research Area

DATA IMAGES

Anti-RPA14 antibody [14.2] used in Western Blot (WB). GTX70240

GTX70240 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
15% SDS PAGE
GTX70240 diluted at 1:500

REFERENCE

Application Reference

Fan JH et al. Biochemistry 2009; 48 (5) : 1099-111 Circular dichroism spectra and electrophoretic mobility shift assays show that human replication protein A binds and melts intramolecular G-quadruplex structures.
Application : WB
Sukhodolets KE et al. Mol Cell Biol 2003; 23 (2) : 493-509 The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene.
Application : WB
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Package List Price ($)
$ 329