Detection of human RPA32 protein using GeneTex RPA32 12F3.3 monoclonal antibody (GTX70243) in HeLa (IR treated) whole cell extract. No radiation, 1hr, 2hr, and 4hr exposure corresponding to lanes 1 through 4 respectively.

RPA32 antibody [12F3.3] detects RPA32 protein at DNA damage foci by immunofluorescent analysis.
Sample: 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: RPA32 protein stained by RPA32 antibody [12F3.3] (GTX70243) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

RPA32 antibody detects RPA32 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and blotted with RPA32 antibody (GTX70243) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.