RPA32 antibody [1H10]

Name: RPA32 antibody [1H10]
Brand: GeneTex
SKU: GTX83691
Price: 519.00 USD

Anti-RPA32 antibody [1H10] used in IHC (Paraffin sections) (IHC-P). GTX83691

Anti-RPA32 antibody [1H10] used in Flow cytometry (FACS). GTX83691

Anti-RPA32 antibody [1H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83691

Anti-RPA32 antibody [1H10] used in Western Blot (WB). GTX83691

Anti-RPA32 antibody [1H10] used in Immunoprecipitation (IP). GTX83691

Cat. No. GTX83691

Host	Mouse
Clonality	Monoclonal
Clone Name	1H10
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, IP
Reactivity	Human, Rat, Dog, Monkey
Package	100 μl ($519)

See all RPA32 products

Datasheet
Document

View product citations for antibody GTX83691 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
ICC/IF	1:100
IHC-P	1:50
FACS	1:100
IP	2ug/500ul

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human RPA2 (NP_002937) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication protein A2 , REPA2 , RP-A p32 , RP-A p34 , RPA32

Cellular Localization

Nucleus

Background

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair | it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Database

Gene ID: 6118 RPA2
UniProt: P15927 RPA2

Research Area

DATA IMAGES

GTX83691 IHC-P Image

IHC-P analysis of human ovary tissue using GTX83691 RPA32 antibody [1H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83691 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83691 RPA32 antibody [1H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83691 IHC-P Image

IHC-P analysis of human prostate tissue using GTX83691 RPA32 antibody [1H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83691 FACS Image

FACS analysis of Jurkat cells using GTX83691 RPA32 antibody [1H10].
Red : Primary antibody
Blue : Negative control antibody

GTX83691 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83691 RPA32 antibody [1H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83691 FACS Image

FACS analysis of HEK293T cells transfected with either RPA32 plasmid(Red) or empty vector control plasmid(Blue) using GTX83691 RPA32 antibody [1H10].

GTX83691 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with RPA32 plasmid using GTX83691 RPA32 antibody [1H10].

GTX83691 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX83691 RPA32 antibody [1H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83691 WB Image

WB analysis of HEK293T cells transfected with RPA32 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83691 RPA32 antibody [1H10].
Loading : 5 ug per lane

GTX83691 FACS Image

FACS analysis of HeLa cells using GTX83691 RPA32 antibody [1H10].
Red : Primary antibody
Blue : Negative control antibody

GTX83691 IHC-P Image

IHC-P analysis of human colon tissue using GTX83691 RPA32 antibody [1H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83691 IHC-P Image

IHC-P analysis of human breast tissue using GTX83691 RPA32 antibody [1H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83691 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX83691 RPA32 antibody [1H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83691 IP Image

IP analysis of DDDDK tagged RPA32 overexpressed HEK293T lysate using GTX83691 RPA32 antibody [1H10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

GTX83691 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83691 RPA32 antibody [1H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83691 WB Image

WB analysis of various cell lines using GTX83691 RPA32 antibody [1H10].
Loading : 35 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

RPA32 antibody [1H10]

Cat. No. GTX83691

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX83691 IHC-P Image

GTX83691 IHC-P Image

GTX83691 IHC-P Image

GTX83691 FACS Image

GTX83691 IHC-P Image

GTX83691 FACS Image

GTX83691 ICC/IF Image

GTX83691 IHC-P Image

GTX83691 WB Image

GTX83691 FACS Image

GTX83691 IHC-P Image

GTX83691 IHC-P Image

GTX83691 IHC-P Image

GTX83691 IP Image

GTX83691 IHC-P Image

GTX83691 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.