GeneTex
United States (US)

RPA32 antibody [MA34]

GTX15783_313_ICC-IF_19011519_935.jpg
GTX15783_1042_IHC-P_19011519_161.jpg
GTX15783_1043_IHC-P_19011519_358.jpg
GTX15783_314_ICC-IF_19011519_831.jpg
GTX15783_1535_WB_19011519_272.jpg
GTX15783_315_ICC-IF_19011519_319.jpg
GTX15783_1041_IHC-P_19011519_464.jpg

Cat No. GTX15783

Host Mouse
Clonality Monoclonal
Clone Name MA34
Isotype IgM
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:100-1:1000
IHC-P 1:10-1:100
Not tested in other applications.

Calculated MW

29 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

RPA p34 subunit isolated from HeLa cells.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Replication Protein A2,Repa2,Rp-A P32,Rp-A P34,Rpa32,Rpa2

Cellular Localization

Nucleus,PML body

Background

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES
GTX15783_313_ICC-IF_19011519_935.jpg

GTX15783 ICC/IF Image

ICC/IF analysis of A431 cells using GTX15783 RPA32 antibody [MA34]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4℃

GTX15783_1042_IHC-P_19011519_161.jpg

GTX15783 IHC-P Image

IHC-P analysis of human kidney tissue using GTX15783 RPA32 antibody [MA34].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX15783_1043_IHC-P_19011519_358.jpg

GTX15783 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX15783 RPA32 antibody [MA34].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX15783_314_ICC-IF_19011519_831.jpg

GTX15783 ICC/IF Image

ICC/IF analysis of C6 cells using GTX15783 RPA32 antibody [MA34]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4℃

GTX15783_1535_WB_19011519_272.jpg

GTX15783 WB Image

WB analysis of purified RPA protein using GTX15783 RPA32 antibody [MA34].

GTX15783_315_ICC-IF_19011519_319.jpg

GTX15783 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15783 RPA32 antibody [MA34]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4℃

GTX15783_1041_IHC-P_19011519_464.jpg

GTX15783 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX15783 RPA32 antibody [MA34].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Package List Price ($)
$ 319