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RPA32 antibody [RPA34-19]

Cat. No. GTX16855

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 RPA34-19

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Hamster
Package
50 μg ($399)
See all RPA32 products

APPLICATION

Application Note

For IF: Use at a concentration of 1 - 5 μg/ml. For IHC-P: Use at a concentration of 2.5 - 5 μg/ml. For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 5 μg/ml. Predicted molecular weight: RPA, is a multisubunit protein containing a 70 kDa, 34 kDa, and 11 kDa protein. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.05M Sodium Phosphate, 0.2% Gelatin

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Tissue / cell preparation (Human); purified from U293 cells.

Purification

Protein G affinity purified

Conjugation

Unconjugated

RRID

AB_384563

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication protein A2 , REPA2 , RP-A p32 , RP-A p34 , RPA32

Cellular Localization

Nucleus

Background

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]

Database

Research Area

REFERENCE

Application Reference

Gatei M et al. Hum Mol Genet 2014; RAD50 phosphorylation promotes ATR downstream signaling and DNA restart following replication stress.
Application : WB
Reactivity : Human
Tuul M et al. PLoS One 2013; 8 (2) : e55361 Rad9, Rad17, TopBP1 and claspin play essential roles in heat-induced activation of ATR kinase and heat tolerance.
Application : ICC/IF
Reactivity : Human
Bartkova J et al. Nature 2006; 444 (7119) : 633-7 Oncogene-induced senescence is part of the tumorigenesis barrier imposed by DNA damage checkpoints.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399