Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using RPA 32 kDa subunit (GTX113004) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

RPA32 antibody detects RPA32 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPA32 stained by RPA32 antibody (GTX113004) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RPA32 antibody (GTX113004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

RPA32 antibody detects RPA2 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
RPA32 antibody (GTX113004) dilution: 1:1000

RPA32 antibody detects RPA2 protein by Western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
12 % SDS-PAGE
RPA32 antibody (GTX113004) dilution: 1:1000