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RPA32 antibody

Anti-RPA32 antibody used in Western Blot (WB). GTX113004
Anti-RPA32 antibody used in Western Blot (WB). GTX113004
Anti-RPA32 antibody used in Western Blot (WB). GTX113004
Anti-RPA32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113004
Anti-RPA32 antibody used in IHC (Paraffin sections) (IHC-P). GTX113004

Cat. No. GTX113004

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RPA32 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

BCL-1 , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPA32. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951747

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication protein A2 , REPA2 , RP-A p32 , RP-A p34 , RPA32

Cellular Localization

Nucleus

Background

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Database

Research Area

DATA IMAGES

Anti-RPA32 antibody used in Western Blot (WB). GTX113004

GTX113004 WB Image

RPA32 antibody detects RPA2 protein by Western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
12 % SDS-PAGE
RPA32 antibody (GTX113004) dilution: 1:1000

Anti-RPA32 antibody used in Western Blot (WB). GTX113004

GTX113004 WB Image

RPA32 antibody detects RPA2 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
RPA32 antibody (GTX113004) dilution: 1:1000

Anti-RPA32 antibody used in Western Blot (WB). GTX113004

GTX113004 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RPA32 antibody (GTX113004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPA32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113004

GTX113004 ICC/IF Image

RPA32 antibody detects RPA32 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPA32 stained by RPA32 antibody (GTX113004) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-RPA32 antibody used in IHC (Paraffin sections) (IHC-P). GTX113004

GTX113004 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using RPA 32 kDa subunit (GTX113004) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Doyle RN et al. bioRxiv 2023; NSC95397 is a Novel HIV Latency Reversing Agent.
Parisotto M et al. J Exp Med 2018; 215 (6) : 1749-1763 PTEN deletion in luminal cells of mature prostate induces replication stress and senescence in vivo.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169