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RPA32 antibody

Anti-RPA32 antibody used in IHC (Paraffin sections) (IHC-P). GTX32844
Anti-RPA32 antibody used in Western Blot (WB). GTX32844
Anti-RPA32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32844
Anti-RPA32 antibody used in Immunoprecipitation (IP). GTX32844

Cat No. GTX32844

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 80-270 of human RPA2 (NP_002937.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

replication protein A2 , REPA2 , RP-A p32 , RP-A p34 , RPA32

Cellular Localization

Nucleus

Background

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-RPA32 antibody used in IHC (Paraffin sections) (IHC-P). GTX32844

GTX32844 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX32844 RPA32 antibody.
Dilution : 1:100

Anti-RPA32 antibody used in Western Blot (WB). GTX32844

GTX32844 WB Image

WB analysis of various samples using GTX32844 RPA32 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RPA32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32844

GTX32844 ICC/IF Image

ICC/IF analysis of U2OS cell expressing GFP tagged RNF168 protein using GTX32844 RPA32 antibody.RNF168 (GFP) can be used to mark cells damage by UV-A treatment.
Green : RNF168
Blue : DAPI

Anti-RPA32 antibody used in Immunoprecipitation (IP). GTX32844

GTX32844 IP Image

IP analysis of Jurkat cell lysate using GTX32844 RPA32 antibody.
IP reaction : 1μg antibody / 200μg cell lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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Package List Price ($)
$ 329