GeneTex
United States (US)

RPA32 antibody

GTX32844_20180530_IHC-P_18121410_630.jpg
GTX32844_20180530_WB_18121410_427.jpg
GTX32844_20180530_ICCIF_18121410_763.jpg
GTX32844_20180530_IP_18121410_357.jpg

Cat No. GTX32844

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 80-270 of human RPA2 (NP_002937.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Replication Protein A2,Repa2,Rp-A P32,Rp-A P34,Rpa32,Rpa2

Database

Research Area

DATA IMAGES
GTX32844_20180530_IHC-P_18121410_630.jpg

GTX32844 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX32844 RPA32 antibody.
Dilution : 1:100

GTX32844_20180530_WB_18121410_427.jpg

GTX32844 WB Image

WB analysis of various samples using GTX32844 RPA32 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32844_20180530_ICCIF_18121410_763.jpg

GTX32844 ICC/IF Image

ICC/IF analysis of U2OS cell expressing GFP tagged RNF168 protein using GTX32844 RPA32 antibody.RNF168 (GFP) can be used to mark cells damage by UV-A treatment.
Green : RNF168
Blue : DAPI

GTX32844_20180530_IP_18121410_357.jpg

GTX32844 IP Image

IP analysis of Jurkat cell lysate using GTX32844 RPA32 antibody.
IP reaction : 1μg antibody / 200μg cell lysate
Dilution : 1:1000

Package List Price ($)
$ 329