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RPA32 antibody

Anti-RPA32 antibody used in Western Blot (WB). GTX70242
Anti-RPA32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70242

Cat No. GTX70242

Host

Mouse

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

For WB: Use at 1:500-1:1000. For ICC/IF: Use at 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

29 kDa. ( Note )

Positive Control

MCF7, T24

PROPERTIES

Form

Liquid

Buffer

Antiserum diluted in PBS pH 7.4, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Full-length human RPA-32 expressed in E. coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Replication Protein A2 , Repa2 , Rp-A P32 , Rp-A P34 , Rpa32 , Rpa2

Background

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase. The middle subunit, RPA-32, is phosphorylated during the S-phase of the cell cycle, in response to DNA damage and during apoptotsis of Jurkat T- lymphocytes.

Database

Research Area

DATA IMAGES

Anti-RPA32 antibody used in Western Blot (WB). GTX70242

GTX70242 WB Image

Detection of human RPA32 protein using GeneTex RPA32 mouse polyclonal antiserum (GTX70242) shown in lane 1 and GeneTex RPA32 rabbit polyclonal antiserum (GTX70258) shown in lane 2. Western Blot was done using HeLa whole cell extract.

Anti-RPA32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70242

GTX70242 ICC/IF Image

RPA32 antibody detects RPA32 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPA32 protein stained by RPA32 antibody (GTX70242) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 329