APPLICATION
Application Note
For WB: Use at 1:500-1:1000. For ICC/IF: Use at 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Positive Control
MCF7, T24
PROPERTIES
Form
Liquid
Buffer
Serum diluted with PBS pH7.4
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Full-length human RPA-32 expressed in E. coli.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_372936
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
replication protein A2 , REPA2 , RP-A p32 , RP-A p34 , RPA32
Cellular Localization
Nucleus
Background
This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]
Database
Research Area
DATA IMAGES
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GTX70242 WB Image
Detection of human RPA32 protein using GeneTex RPA32 mouse polyclonal antiserum (GTX70242) shown in lane 1 and GeneTex RPA32 rabbit polyclonal antiserum (GTX70258) shown in lane 2. Western Blot was done using HeLa whole cell extract.
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GTX70242 ICC/IF Image
RPA32 antibody detects RPA32 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPA32 protein stained by RPA32 antibody (GTX70242) diluted at 1:500. Blue: Hoechst 33342 staining.
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REFERENCE
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REVIEW
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