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RPA32 antibody

Anti-RPA32 antibody used in Western Blot (WB). GTX70258
Anti-RPA32 antibody used in Western Blot (WB). GTX70258
Anti-RPA32 antibody used in Western Blot (WB). GTX70258
Anti-RPA32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70258
Anti-RPA32 antibody used in Western Blot (WB). GTX70258

Cat No. GTX70258

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

Suggested starting dilutions are as follows: WB: 1:2500, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

29 kDa. ( Note )

Positive Control

HeLa, Raji, 3T3

PROPERTIES

Form

Liquid

Buffer

Antiserum diluted in PBS pH 7.4, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Full-length human RPA-32 expressed in E. coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Replication Protein A2 , Repa2 , Rp-A P32 , Rp-A P34 , Rpa32 , Rpa2

Cellular Localization

Nuclear

Background

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase. The middle subunit, RPA-32, is phosphorylated during the S-phase of the cell cycle, in response to DNA damage and during apoptotsis of Jurkat T- lymphocytes.

Database

Research Area

DATA IMAGES

Anti-RPA32 antibody used in Western Blot (WB). GTX70258

GTX70258 WB Image

Detection of human RPA32 protein using GeneTex RPA32 polyclonal antiserum (GTX70258) in HeLa whole cell extract.

Anti-RPA32 antibody used in Western Blot (WB). GTX70258

GTX70258 WB Image

The WB analysis of RPA32 antibody was published by Adeyemi RO and colleagues in the journal PLoS Pathog in 2010.PMID: 20949077

Anti-RPA32 antibody used in Western Blot (WB). GTX70258

GTX70258 WB Image

The WB analysis of RPA32 antibody was published by Adeyemi RO and colleagues in the journal PLoS Pathog in 2010.PMID: 20949077

Anti-RPA32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70258

GTX70258 ICC/IF Image

RPA32 antibody detects RPA32 protein at DNA damage foci by immunofluorescent analysis.
Sample: 2mM Hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: RPA32 protein stained by RPA32 antibody (GTX70258) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-RPA32 antibody used in Western Blot (WB). GTX70258

GTX70258 WB Image

GTXat a dilution of 1:2500 staining RPA 32 in A) HeLa whole cell lysate, B) Raji whole cell lysate and C) NIH-3T3 whole cell lysate by Western Blot (ECL)

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329