RPA32 (phospho Ser4) antibody [HL5883]

RPA32 (phospho Ser4) antibody [HL5883] detects RPA32 (phospho Ser4) protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: RPA32 (phospho Ser4) stained by RPA32 (phospho Ser4) antibody [HL5883] (GTX647207) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with RPA32 (phospho Ser4) antibody [HL5883] (GTX647207) diluted at 1:1000 and RPA32 antibody (GTX113004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with RPA32 (phospho Ser4) antibody [HL5883] (GTX647207) diluted at 1:1000 and RPA32 antibody (GTX113004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.