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RPA70 antibody [8C3-D12-H10]

Anti-RPA70 antibody [8C3-D12-H10] used in Western Blot (WB). GTX49157
Anti-RPA70 antibody [8C3-D12-H10] used in Western Blot (WB). GTX49157

Cat. No. GTX49157

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8C3-D12-H10

Isotype

 IgG2a

Application

 WB

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μg ($399)
See all RPA70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human RPA70 protein fragments expressed in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication protein A1 , HSSB , MST075 , REPA1 , RF-A , RP-A , RPA70

Cellular Localization

Nucleus

Background

This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-RPA70 antibody [8C3-D12-H10] used in Western Blot (WB). GTX49157

GTX49157 WB Image

WB analysis of indicated lysates using RPA70 antibody at a dilution of 1:1,000.

Anti-RPA70 antibody [8C3-D12-H10] used in Western Blot (WB). GTX49157

GTX49157 WB Image

WB analysis of indicated lysates using RPA70 antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399