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RPB1 CTD (phospho Ser5) antibody [GT1303]

Recombinant antibody Orthogonal
Anti-RPB1 CTD (phospho Ser5) antibody [GT1303] used in Western Blot (WB). GTX03215
Anti-RPB1 CTD (phospho Ser5) antibody [GT1303] used in Western Blot (WB). GTX03215
Anti-RPB1 CTD (phospho Ser5) antibody [GT1303] used in Western Blot (WB). GTX03215

Cat. No. GTX03215

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1303

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all RPB1 CTD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

217 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S5 of human POLR2A carboxy-terminal domain (CTD) heptapeptide repeat YSPTSPS.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA polymerase II subunit A , POLR2 , POLRA , RPB1 , RPBh1 , RPO2 , RPOL2 , RpIILS , hRPB220 , hsRPB1

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RPB1 CTD (phospho Ser5) antibody [GT1303] used in Western Blot (WB). GTX03215

GTX03215 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB1 CTD (phospho Ser5) antibody [GT1303] (GTX03215) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPB1 CTD (phospho Ser5) antibody [GT1303] used in Western Blot (WB). GTX03215

GTX03215 WB Image

Orthogonal

WB analysis of MCF7 cells were treated by Doxorubicin (0.5 uM) at 37ºC for 24 hours or treated by nocodazole (50 ng/mL) at 37ºC for 20 hours or treated by CIP(20uL/400ul) at 37ºC for 1 hour using GTX03215 RPB1 CTD (phospho Ser5) antibody [GT1303].
Dilution : 1:1000
Loading : 25μg per lane

Anti-RPB1 CTD (phospho Ser5) antibody [GT1303] used in Western Blot (WB). GTX03215

GTX03215 WB Image

Orthogonal

WB analysis of C2C12 cells were treated by CIP(20uL/400ul) at 37ºC for 1 hour using GTX03215 RPB1 CTD (phospho Ser5) antibody [GT1303].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 419