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RPB1 antibody [4H8]

Anti-RPB1 antibody [4H8] used in Flow cytometry (FACS). GTX80341
Anti-RPB1 antibody [4H8] used in Western Blot (WB). GTX80341
Anti-RPB1 antibody [4H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80341
Anti-RPB1 antibody [4H8] used in Western Blot (WB). GTX80341
Anti-RPB1 antibody [4H8] used in Flow cytometry (FACS). GTX80341

Cat. No. GTX80341

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4H8

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ChIP assay

Reactivity

 Human, Japanese Medaka
Package
50 μl ($639)
See all RPB1 products

APPLICATION

Application Note

Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

217 kDa. ( Note )

Product Note

Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recognize UN-phosphorylated repeats as there might conceivably be a few of those even in the II0/hyper-phos RPB1 form.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_11170234

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA polymerase II subunit A , POLR2 , POLRA , RPB1 , RPBh1 , RPO2 , RPOL2 , RpIILS , hRPB220 , hsRPB1

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RPB1 antibody [4H8] used in Flow cytometry (FACS). GTX80341

GTX80341 FACS Image

FACS analysis of Daudi cells using GTX80341 RPB1 antibody [4H8]
Orange: Negative control
Blue: Daudi cells

Anti-RPB1 antibody [4H8] used in Western Blot (WB). GTX80341

GTX80341 WB Image

WB analysis of various samples using GTX80341 RPB1 antibody [4H8]
Lane1: Hela
Lane2: MCF7
Lane3: NIH-3T3
Lane4: Cos7

Anti-RPB1 antibody [4H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80341

GTX80341 ICC/IF Image

ICC/IF analysis of Hela cells using GTX80341 RPB1 antibody [4H8]
Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Anti-RPB1 antibody [4H8] used in Western Blot (WB). GTX80341

GTX80341 WB Image

ICC/IF analysis of Hela cells using GTX80341 MCF7 antibody [4H8]

Anti-RPB1 antibody [4H8] used in Flow cytometry (FACS). GTX80341

GTX80341 FACS Image

FACS analysis of Hela cells using GTX80341 RPB1 antibody [4H8]
Orange: Negative control
Blue: Hela cells

REFERENCE

Application Reference

Hagiwara A et al. Biol Reprod 2014; Luteinizing Hormone-Induced Expression of Ptger4b, a Prostaglandin E2 Receptor Indispensable for Ovulation of the Medaka Oryzias latipes, Is Regulated by a Genomic Mechanism Involving Nuclear Progestin Receptor.
Application : ChIP assay
Reactivity : Japanese Medaka
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639
Custom Conjugations