GeneTex
  • Country / Location Selection

United States (US)

RPB2 antibody [HL1042]

Recombinant antibody
Anti-RPB2 antibody [HL1042] used in IHC (Paraffin sections) (IHC-P). GTX635976
Anti-RPB2 antibody [HL1042] used in Western Blot (WB). GTX635976
Anti-RPB2 antibody [HL1042] used in Western Blot (WB). GTX635976
Anti-RPB2 antibody [HL1042] used in Western Blot (WB). GTX635976

Cat No. GTX635976

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1042

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909928

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RNA polymerase II subunit B , POL2RB , RPB2 , hRPB140

Cellular Localization

Nucleus

Background

This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-RPB2 antibody [HL1042] used in IHC (Paraffin sections) (IHC-P). GTX635976

GTX635976 IHC-P Image

RPB2 antibody [HL1042] detects RPB2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RPB2 stained by RPB2 antibody [HL1042] (GTX635976) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPB2 antibody [HL1042] used in Western Blot (WB). GTX635976

GTX635976 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPB2 antibody [HL1042] used in Western Blot (WB). GTX635976

GTX635976 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPB2 antibody [HL1042] used in Western Blot (WB). GTX635976

GTX635976 WB Image

293T whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for RPB2 antibody [HL1042] (GTX635976). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RPB2 antibody [HL1042] (GTX635976). Be the first to share your experience with this product.
Package List Price ($)
$ 369
$ 169
Custom Conjugations