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RPB2 antibody [HL1043]

Recombinant antibody
Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977
Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977
Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977
Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977
Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977
Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977
Anti-RPB2 antibody [HL1043] used in IHC (Whole mount) (IHC-Wm). GTX635977
Anti-RPB2 antibody [HL1043] used in IHC (Whole mount) (IHC-Wm). GTX635977
Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977
Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977

Cat. No. GTX635977

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1043

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all RPB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Predict Reactivity

Drosophila, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909929

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA polymerase II subunit B , POL2RB , RPB2 , hRPB140

Cellular Localization

Nucleus

Background

This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977

GTX635977 IHC-P Image

RPB2 antibody [HL1043] detects RPB2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
RPB2 stained by RPB2 antibody [HL1043] (GTX635977) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977

GTX635977 IHC-P Image

RPB2 antibody [HL1043] detects RPB2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
RPB2 stained by RPB2 antibody [HL1043] (GTX635977) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977

GTX635977 IHC-P Image

RPB2 antibody [HL1043] detects RPB2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RPB2 stained by RPB2 antibody [HL1043] (GTX635977) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977

GTX635977 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (GTX635977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977

GTX635977 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (GTX635977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977

GTX635977 WB Image

293T whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (GTX635977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPB2 antibody [HL1043] used in IHC (Whole mount) (IHC-Wm). GTX635977

GTX635977 IHC-Wm Image

RPB2 antibody [HL1043] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: RPB2 stained by RPB2 antibody [HL1043] (GTX635977) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-RPB2 antibody [HL1043] used in IHC (Whole mount) (IHC-Wm). GTX635977

GTX635977 IHC-Wm Image

RPB2 antibody [HL1043] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: RPB2 stained by RPB2 antibody [HL1043] (GTX635977) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977

GTX635977 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (GTX635977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977

GTX635977 WB Image

Whole zebrafish extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (GTX635977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations