GeneTex
United States (US)

RPB2 antibody [N1], N-term

GTX102535_39918_IHC_19011506_623.jpg
GTX102535_WB_19011506_786.jpg
GTX102535_39918_WB_M_19011506_835.jpg
GTX102535_39918_20151227_IHC-P_19011506_149.jpg
GTX102535_IFA_19011506_269.jpg

Cat No. GTX102535

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Positive Control

H1299 , BCL-1

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

POL2RB antibody, RPB2 antibody, hRPB140 antibody, hsRPB2 antibody, POLR2B antibody, DNA-directed RNA polymerase II 140 kDa polypeptide antibody, DNA-directed RNA polymerase II subunit B antibody, "DNA directed RNA polymerase II 140 kDa polypeptide, RNA polymerase II subunit 2, RNA polymerase II second largest subunit antibody", RNA polymerase II subunit B2 antibody, RNA polymerase II subunit 2 antibody, DNA-directed RNA polymerase II subunit RPB2 antibody, "polymerase (RNA) II (DNA directed) polypeptide B, 140kDa antibody"

Cellular Localization

Nucleus

Background

This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq]
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Database

DATA IMAGES
GTX102535_39918_IHC_19011506_623.jpg

GTX102535 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using RPB2(GTX102535) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102535_WB_19011506_786.jpg

GTX102535 WB Image

Sample (30 µg of whole cell lysate)
A: H1299
5% SDS PAGE
GTX102535 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102535_39918_WB_M_19011506_835.jpg

GTX102535 WB Image

Sample (30 µg of whole cell lysate)
A: BCL-1
5% SDS PAGE
GTX102535 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102535_39918_20151227_IHC-P_19011506_149.jpg

GTX102535 IHC-P Image

RPB2 antibody [N1], N-term detects RPB2 protein at nucleus in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RPB2 antibody [N1], N-term (GTX102535) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102535_IFA_19011506_269.jpg

GTX102535 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPB2(GTX102535) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169