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RPB2 antibody [N1], N-term

Anti-RPB2 antibody [N1], N-term used in Western Blot (WB). GTX102535
Anti-RPB2 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102535
Anti-RPB2 antibody [N1], N-term used in Western Blot (WB). GTX102535
Anti-RPB2 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX102535
Anti-RPB2 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX102535
Anti-RPB2 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX102535

Cat. No. GTX102535

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RPB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Positive Control

H1299 , BCL-1

Predict Reactivity

Rat, Zebrafish, Drosophila, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951313

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA polymerase II subunit B , POL2RB , RPB2 , hRPB140

Cellular Localization

Nucleus

Background

This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPB2 antibody [N1], N-term used in Western Blot (WB). GTX102535

GTX102535 WB Image

Sample (30 μg of whole cell lysate)
A: BCL-1
5% SDS PAGE
GTX102535 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPB2 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102535

GTX102535 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPB2(GTX102535) antibody at 1:200 dilution.

Anti-RPB2 antibody [N1], N-term used in Western Blot (WB). GTX102535

GTX102535 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
5% SDS PAGE
GTX102535 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPB2 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX102535

GTX102535 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using RPB2(GTX102535) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPB2 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX102535

GTX102535 IHC-P Image

RPB2 antibody [N1], N-term detects RPB2 protein at nucleus in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RPB2 antibody [N1], N-term (GTX102535) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPB2 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX102535

GTX102535 IHC-P Image

RPB2 antibody [N1], N-term detects RPB2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tongue.
RPB2 stained by RPB2 antibody [N1], N-term (GTX102535) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Cobo I et al. Immunity 2022; 55 (8) : 1386-1401.e10 DNA methyltransferase 3 alpha and TET methylcytosine dioxygenase 2 restrain mitochondrial DNA-mediated interferon signaling in macrophages.
Marten A Hoeksema et al. Sci Adv 2021; 7 (25) Mechanisms underlying divergent responses of genetically distinct macrophages to IL-4.
Application : ChIP assay
Reactivity : Mouse
J?nsson ME et al. Nat Commun 2019; 10 (1) : 3182 Activation of neuronal genes via LINE-1 elements upon global DNA demethylation in human neural progenitors.
Application : ChIP assay
Reactivity : Human
Heinz S et al. Cell 2018; 174 (6) : 1522-1536.e22 Transcription Elongation Can Affect Genome 3D Structure.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169