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RPB2 antibody

Anti-RPB2 antibody used in Western Blot (WB). GTX64604
Anti-RPB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64604
Anti-RPB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64604
Anti-RPB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64604

Cat. No. GTX64604

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all RPB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RNA polymerase II subunit B , POL2RB , RPB2 , hRPB140

Cellular Localization

Nucleus

Background

This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-RPB2 antibody used in Western Blot (WB). GTX64604

GTX64604 WB Image

WB analysis of various sample lysates using GTX64604 RPB2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RPB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64604

GTX64604 IHC-P Image

IHC-P analysis of rat brain tissue using GTX64604 RPB2 antibody.
Dilution : 1:100

Anti-RPB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64604

GTX64604 IHC-P Image

IHC-P analysis of rat liver tissue using GTX64604 RPB2 antibody.
Dilution : 1:100

Anti-RPB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64604

GTX64604 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX64604 RPB2 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399