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RPB8 antibody

Anti-RPB8 antibody used in Immunoprecipitation (IP). GTX65882
Anti-RPB8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65882
Anti-RPB8 antibody used in Western Blot (WB). GTX65882

Cat. No. GTX65882

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all RPB8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-150 of human POLR2H (NP_006223.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA polymerase II subunit H , RPABC3 , RPB17 , RPB8

Cellular Localization

Nucleolus

Background

The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Database

DATA IMAGES

Anti-RPB8 antibody used in Immunoprecipitation (IP). GTX65882

GTX65882 IP Image

IP analysis of MCF-7 cell lysate using GTX65882 RPB8 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-RPB8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65882

GTX65882 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX65882 RPB8 antibody.
Blue : DAPI

Anti-RPB8 antibody used in Western Blot (WB). GTX65882

GTX65882 WB Image

WB analysis of various sample lysates using GTX65882 RPB8 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399