GeneTex

  • Country / Location Selection

United States (US)

RPE65 antibody [401.8B11.3D9]

Orthogonal
Anti-RPE65 antibody [401.8B11.3D9] used in IHC (Frozen sections) (IHC-Fr). GTX13826
Anti-RPE65 antibody [401.8B11.3D9] used in Western Blot (WB). GTX13826
Anti-RPE65 antibody [401.8B11.3D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13826
Anti-RPE65 antibody [401.8B11.3D9] used in IHC (Paraffin sections) (IHC-P). GTX13826

Cat. No. GTX13826

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 401.8B11.3D9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, MS

Reactivity

 Human, Mouse, Rat, Bovine, Dog, Chicken, Pig, Xenopus
Package
100 μl ($489)
See all RPE65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:10 - 1:500
IHC-P 1:250 - 1:500
IHC-Fr 1:250
FACS Assay dependent
MS Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Predict Reactivity

Duck(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Bovine RPE65 microsomal membrane proteins. [UniProt# Q28175]

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372073

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

retinoid isomerohydrolase RPE65

Cellular Localization

Cytoplasm,Cell membrane

Background

Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.

Database

Research Area

DATA IMAGES

Anti-RPE65 antibody [401.8B11.3D9] used in IHC (Frozen sections) (IHC-Fr). GTX13826

GTX13826 IHC-Fr Image

IHC-Fr analysis of mouse retina tissue using GTX13826 RPE65 antibody [401.8B11.3D9].

Anti-RPE65 antibody [401.8B11.3D9] used in Western Blot (WB). GTX13826

GTX13826 WB Image

Orthogonal

WB analysis of 20μg RPE65 overexpressing COS-7 cell (lane 1) and 5μg of bovine retinal pigment epithelium membrane fraction (lane 2) using GTX13826 RPE65 antibody [401.8B11.3D9].

Anti-RPE65 antibody [401.8B11.3D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13826

GTX13826 ICC/IF Image

ICC/IF analysis of ARPE-19 cells using GTX13826 RPE65 antibody [401.8B11.3D9].
Dilution : 1:100
Fixation : 4% PFA
Permibilization : 0.2% Triton X-100

Anti-RPE65 antibody [401.8B11.3D9] used in IHC (Paraffin sections) (IHC-P). GTX13826

GTX13826 IHC-P Image

IHC-P analysis of human glioblastoma tissue using GTX13826 RPE65 antibody [401.8B11.3D9].
Dilution : 1:500
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)

REFERENCE

Application Reference

Wang Y et al. J Mol Cell Biol 2023; 15 (3) METTL3-mediated m6A modification of HMGA2 mRNA promotes subretinal fibrosis and epithelial-mesenchymal transition.
Moshfegh Y et al. Hum Mol Genet 2016; (Epub) BESTROPHIN1 mutations cause defective chloride conductance in patient stem cell-derived RPE.
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for RPE65 antibody [401.8B11.3D9] (GTX13826). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 489