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RPL11 antibody

Anti-RPL11 antibody used in IHC (Whole mount) (IHC-Wm). GTX101651
Anti-RPL11 antibody used in Western Blot (WB). GTX101651
Anti-RPL11 antibody used in IHC (Paraffin sections) (IHC-P). GTX101651
Anti-RPL11 antibody used in Western Blot (WB). GTX101651

Cat. No. GTX101651

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all RPL11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

HeLa , Mouse liver

Predict Reactivity

Rat, Drosophila, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175592

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L11 , DBA7 , GIG34 , L11 , uL5

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternative splice variants encoding different isoforms may exist, but they have not been fully characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPL11 antibody used in IHC (Whole mount) (IHC-Wm). GTX101651

GTX101651 IHC-Wm Image

RPL11 antibody detects Rpl11 protein on whole-mount zebrafish embryos by immunohistochemical analysis.?
Sample: Paraformaldehyde-fixed zebrafish embryos.
RPL11 antibody (GTX101651) dilution: 1:200.

Anti-RPL11 antibody used in Western Blot (WB). GTX101651

GTX101651 WB Image

Sample (50 ug of whole cell lysate)
A: mouse Liver
12% SDS PAGE
GTX101651 diluted at 1:1000

Anti-RPL11 antibody used in IHC (Paraffin sections) (IHC-P). GTX101651

GTX101651 IHC-P Image

RPL11 antibody detects RPL11 protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
RPL11 antibody (GTX101651) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL11 antibody used in Western Blot (WB). GTX101651

GTX101651 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX101651 diluted at 1:1000

REFERENCE

Application Reference

Fregoso OI et al. Mol Cell 2013; 50 (1) : 56-66 Splicing-factor oncoprotein SRSF1 stabilizes p53 via RPL5 and induces cellular senescence.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169