GeneTex
United States (US)

RPL11 antibody

GTX101651_40744_WB_19011506_913.jpg
GTX101651_40744_IHC_19011506_892.jpg
GTX101651_40744_WB_M_Liver_19011506_667.jpg

Cat No. GTX101651

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

HeLa , mouse Liver

Predict Reactivity

Rat, Zebrafish, Drosophila, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DBA7 antibody, GIG34 antibody, RPL11 antibody, CLL-associated antigen KW-12 antibody, 60S ribosomal protein L11 antibody, cell growth-inhibiting protein 34 antibody, ribosomal protein L11 antibody

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternative splice variants encoding different isoforms may exist, but they have not been fully characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

DATA IMAGES
GTX101651_40744_WB_19011506_913.jpg

GTX101651 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX101651 diluted at 1:1000

GTX101651_40744_IHC_19011506_892.jpg

GTX101651 IHC-P Image

RPL11 antibody detects RPL11 protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
RPL11 antibody (GTX101651) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101651_40744_WB_M_Liver_19011506_667.jpg

GTX101651 WB Image

Sample (50 ug of whole cell lysate)
A: mouse Liver
12% SDS PAGE
GTX101651 diluted at 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169