APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , HeLa
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.86 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human RPL13A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951749
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ribosomal protein L13a , L13A , TSTA1
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative polyA signals have been observed. This gene is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in its second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-RPL13A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102228](/templates/default/js/lightbox/images/loading.gif "Anti-RPL13A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102228")
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GTX102228 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using RPL13A antibody [N1C3] (GTX102228) at 1:300 dilution.
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GTX102228 WB Image
Sample (30 μg of whole cell lysate)
A: zebrafish heart
12% SDS PAGE
GTX102228 diluted at 1:500
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GTX102228 WB Image
Sample (30 ug of whole cell lysate)
A:293T
B:HeLa
10% SDS PAGE
GTX102228 diluted at 1:500
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GTX102228 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPL13A(GTX102228) antibody at 1:200 dilution.
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GTX102228 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using RPL13A(GTX102228) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for RPL13A antibody [N1C3] (GTX102228). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RPL13A antibody [N1C3] (GTX102228). Be the first to share your experience with this product.
![Anti-RPL13A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102228](/upload/website/prouct_img/small/GTX102228/GTX102228_40023_IHC_Z_22111423_467.webp "Anti-RPL13A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102228")
![Anti-RPL13A antibody [N1C3] used in Western Blot (WB). GTX102228](/upload/website/prouct_img/small/GTX102228/GTX102228_40023_WB_Z_22111423_753.webp "Anti-RPL13A antibody [N1C3] used in Western Blot (WB). GTX102228")
![Anti-RPL13A antibody [N1C3] used in Western Blot (WB). GTX102228](/upload/website/prouct_img/small/GTX102228/GTX102228_WB_w_23060100_630.webp "Anti-RPL13A antibody [N1C3] used in Western Blot (WB). GTX102228")
![Anti-RPL13A antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102228](/upload/website/prouct_img/small/GTX102228/GTX102228_IFA_w_23060100_457.webp "Anti-RPL13A antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102228")
![Anti-RPL13A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102228](/upload/website/prouct_img/small/GTX102228/GTX102228_40023_IHC_w_23060100_993.webp "Anti-RPL13A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102228")
![Anti-RPL13A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102228](/upload/website/prouct_img/normal/GTX102228/GTX102228_40023_IHC_Z_22111423_467.webp "Anti-RPL13A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102228")
![Anti-RPL13A antibody [N1C3] used in Western Blot (WB). GTX102228](/upload/website/prouct_img/normal/GTX102228/GTX102228_40023_WB_Z_22111423_753.webp "Anti-RPL13A antibody [N1C3] used in Western Blot (WB). GTX102228")
![Anti-RPL13A antibody [N1C3] used in Western Blot (WB). GTX102228](/upload/website/prouct_img/normal/GTX102228/GTX102228_WB_w_23060100_630.webp "Anti-RPL13A antibody [N1C3] used in Western Blot (WB). GTX102228")
![Anti-RPL13A antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102228](/upload/website/prouct_img/normal/GTX102228/GTX102228_IFA_w_23060100_457.webp "Anti-RPL13A antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102228")
![Anti-RPL13A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102228](/upload/website/prouct_img/normal/GTX102228/GTX102228_40023_IHC_w_23060100_993.webp "Anti-RPL13A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102228")