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RPL13A antibody [N1C3]

Anti-RPL13A antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102228
Anti-RPL13A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102228
Anti-RPL13A antibody [N1C3] used in Western Blot (WB). GTX102228

Cat No. GTX102228

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL13A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ribosomal protein L13a , L13A , TSTA1

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative polyA signals have been observed. This gene is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in its second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-RPL13A antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102228

GTX102228 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPL13A(GTX102228) antibody at 1:200 dilution.

Anti-RPL13A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102228

GTX102228 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using RPL13A(GTX102228) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL13A antibody [N1C3] used in Western Blot (WB). GTX102228

GTX102228 WB Image

Sample (30 ug of whole cell lysate)
A:293T
B:HeLa
10% SDS PAGE
GTX102228 diluted at 1:500

REFERENCE
There are currently no references for RPL13A antibody [N1C3] (GTX102228). Be the first to share your publications with this product.
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169