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RPL14 antibody

Anti-RPL14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33473
Anti-RPL14 antibody used in Western Blot (WB). GTX33473
Anti-RPL14 antibody used in IHC (Paraffin sections) (IHC-P). GTX33473

Cat. No. GTX33473

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all RPL14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RPL14 (NP_003964.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L14 , CAG-ISL-7 , CTG-B33 , L14 , RL14 , hRL14

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-RPL14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33473

GTX33473 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX33473 RPL14 antibody.
Blue : DAPI

Anti-RPL14 antibody used in Western Blot (WB). GTX33473

GTX33473 WB Image

WB analysis of various sample lysates using GTX33473 RPL14 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RPL14 antibody used in IHC (Paraffin sections) (IHC-P). GTX33473

GTX33473 IHC-P Image

IHC-P analysis of rat liver tissue using GTX33473 RPL14 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399