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RPL17 antibody [N1C3-2]

Anti-RPL17 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111934
Anti-RPL17 antibody [N1C3-2] used in Western Blot (WB). GTX111934
Anti-RPL17 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX111934
Anti-RPL17 antibody [N1C3-2] used in Western Blot (WB). GTX111934
Anti-RPL17 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX111934
Anti-RPL17 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX111934

Cat. No. GTX111934

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all RPL17 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

HeLa , HepG2 , rat pancreas

Predict Reactivity

Mouse, Zebrafish, Bovine, Cat, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human RPL17 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951755

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L17 , L17 , PD-1 , RPL23

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17. Two alternative splice variants have been observed, each encoding the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPL17 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111934

GTX111934 ICC/IF Image

RPL17 antibody [N1C3-2] detects RPL17 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPL17 protein stained by RPL17 antibody [N1C3-2] (GTX111934) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
Anti-RPL17 antibody [N1C3-2] used in Western Blot (WB). GTX111934

GTX111934 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
B: HepG2 (GTX27900)
12% SDS PAGE
GTX111934 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPL17 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX111934

GTX111934 IHC-P Image

RPL17 antibody [N1C3-2] detects RPL17 protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
RPL17 antibody [N1C3-2] (GTX111934) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPL17 antibody [N1C3-2] used in Western Blot (WB). GTX111934

GTX111934 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RPL17 antibody [N1C3-2] (GTX111934) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPL17 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX111934

GTX111934 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using RPL17(GTX111934) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPL17 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX111934

GTX111934 IHC-P Image

RPL17 antibody [N1C3-2] detects RPL17 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
RPL17 antibody [N1C3-2] (GTX111934) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Dionne KL et al. J Biol Chem 2019; 294 (6) : 1944-1955 The 40S ribosomal protein uS5 (RPS2) assembles into an extraribosomal complex with human ZNF277 that competes with the PRMT3-uS5 interaction.
Landry-Voyer AM et al. Mol Cell Biol 2016; (Epub) Human PDCD2L is an export substrate of CRM1 that associates with 40S ribosomal subunit precursors.
Application : WB
Reactivity : Human
Fregoso OI et al. Mol Cell 2013; 50 (1) : 56-66 Splicing-factor oncoprotein SRSF1 stabilizes p53 via RPL5 and induces cellular senescence.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169