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RPL29 antibody

Anti-RPL29 antibody used in Western Blot (WB). GTX101833
Anti-RPL29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101833
Anti-RPL29 antibody used in IHC (Paraffin sections) (IHC-P). GTX101833

Cat No. GTX101833

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , U87-MG , SK-N-SH

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL29. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribosomal protein L29 , HIP , HUMRPL29 , L29 , RPL29P10 , RPL29_3_370

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29E family of ribosomal proteins. The protein is also a peripheral membrane protein expressed on the cell surface that directly binds heparin. Although this gene was previously reported to map to 3q29-qter, it is believed that it is located at 3p21.3-p21.2. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

DATA IMAGES

Anti-RPL29 antibody used in Western Blot (WB). GTX101833

GTX101833 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
C: U87-MG
D: SK-N-SH
12% SDS PAGE
GTX101833 diluted at 1:10000

Anti-RPL29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101833

GTX101833 ICC/IF Image

RPL29 antibody detects RPL29 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPL29 protein stained by RPL29 antibody (GTX101833) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-RPL29 antibody used in IHC (Paraffin sections) (IHC-P). GTX101833

GTX101833 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using RPL29(GTX101833) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169