Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RPL3 antibody [N1N3] (GTX101854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunofluorescence analysis of methanol-fixed A549, using RPL3(GTX101854) antibody at 1:500 dilution.