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RPL3 antibody [N3C3]

Orthogonal
Anti-RPL3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114725
Anti-RPL3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114725
Anti-RPL3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114725
Anti-RPL3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114725
Anti-RPL3 antibody [N3C3] used in Western Blot (WB). GTX114725

Cat. No. GTX114725

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RPL3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

Jurkat , Raji

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619064

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L3 , ASC-1 , L3 , TARBP-B

Cellular Localization

Cytoplasm

Background

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene's introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPL3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114725

GTX114725 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using RPL3(GTX114725) antibody at 1:200 dilution.

Anti-RPL3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114725

GTX114725 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 4T1 xenograft, using RPL3(GTX114725) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPL3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114725

GTX114725 IHC-P Image

RPL3 antibody [N3C3] detects RPL3 protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
RPL3 antibody [N3C3] (GTX114725) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPL3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114725

GTX114725 IHC-P Image

RPL3 antibody [N3C3] detects RPL3 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RPL3 antibody [N3C3] (GTX114725) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPL3 antibody [N3C3] used in Western Blot (WB). GTX114725

GTX114725 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RPL3 antibody [N3C3] (GTX114725) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Fellmann F et al. JCI Insight 2024; 9 (17) An atypical form of 60S ribosomal subunit in Diamond-Blackfan anemia linked to RPL17 variants.
Imai H et al. Nucleic Acids Res 2023; 51 (14) : e76 Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification.
Watanabe S et al. J Cell Sci 2018; (Epub) Glutamate-rich WD40 repeat containing 1 regulates ribosomal protein L23 levels via the ubiquitin-proteasome system.
Application : WB
Reactivity : Human
Shi J et al. J Biol Chem 2015; 290 (24) : 14986-5003 Methylxanthines Increase Expression of the Splicing Factor SRSF2 by Regulating Multiple Post-transcriptional Mechanisms.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169