GeneTex
United States (US)

RPL3 antibody [N3C3]

GTX114725_WB_18121410_115.jpg
GTX114725_40219_20150612_IHC_M_2_18121410_342.jpg
GTX114725_40219_IHC_18121410_312.jpg
GTX114725_40219_IFA_18121410_963.jpg
GTX114725_40219_20150612_IHC_M_18121410_466.jpg

Cat No. GTX114725

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

0.74mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC104284 antibody, TARBP-B antibody, RPL3 antibody, "ribosomal protein L3, isoform a antibody", HIV-1 TAR RNA-binding protein B antibody, 60S ribosomal protein L3 antibody, ribosomal protein L3 antibody

Cellular Localization

Cytoplasm

Background

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene's introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]
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Database

DATA IMAGES
GTX114725_WB_18121410_115.jpg

GTX114725 WB Image

Sample (30 µg of whole cell lysate)
A: JurKat
B: Raji
10% SDS PAGE
GTX114725 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114725_40219_20150612_IHC_M_2_18121410_342.jpg

GTX114725 IHC-P Image

RPL3 antibody [N3C3] detects RPL3 protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
RPL3 antibody [N3C3] (GTX114725) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114725_40219_IHC_18121410_312.jpg

GTX114725 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 4T1 xenograft, using RPL3(GTX114725) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114725_40219_IFA_18121410_963.jpg

GTX114725 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using RPL3(GTX114725) antibody at 1:200 dilution.

GTX114725_40219_20150612_IHC_M_18121410_466.jpg

GTX114725 IHC-P Image

RPL3 antibody [N3C3] detects RPL3 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RPL3 antibody [N3C3] (GTX114725) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169