APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.46 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human RPL32 Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886209
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ribosomal protein L32 , L32 , PP9932
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX130214 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody (GTX130214) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX130214 IHC-P Image
RPL32 antibody detects RPL32 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.RPL32 stained by RPL32 antibody (GTX130214) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX130214 IHC-P Image
RPL32 antibody detects RPL32 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.RPL32 stained by RPL32 antibody (GTX130214) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX130214 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL32 antibody (GTX130214) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX130214 ICC/IF Image
RPL32 antibody detects RPL32 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPL32 stained by RPL32 antibody (GTX130214) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
|
|
|
GTX130214 ICC/IF Image
RPL32 antibody detects RPL32 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPL32 protein stained by RPL32 antibody (GTX130214) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
|
|
|
GTX130214 IHC-P Image
RPL32 antibody detects RPL32 protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
RPL32 antibody (GTX130214) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX130214 IHC-P Image
RPL32 antibody detects RPL32 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
RPL32 antibody (GTX130214) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
REFERENCE
REVIEW
There are currently no reviews for RPL32 antibody (GTX130214). Be the first to share your experience with this product.
