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RPL32 antibody

Anti-RPL32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66149
Anti-RPL32 antibody used in Western Blot (WB). GTX66149
Anti-RPL32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66149
Anti-RPL32 antibody used in IHC (Paraffin sections) (IHC-P). GTX66149
Anti-RPL32 antibody used in IHC (Paraffin sections) (IHC-P). GTX66149
Anti-RPL32 antibody used in IHC (Paraffin sections) (IHC-P). GTX66149

Cat. No. GTX66149

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all RPL32 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-135 of human RPL32 (NP_000985.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L32 , L32 , PP9932

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RPL32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66149

GTX66149 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX66149 RPL32 antibody.
Blue : DAPI
Dilution : 1:100

Anti-RPL32 antibody used in Western Blot (WB). GTX66149

GTX66149 WB Image

WB analysis of various sample lysates using GTX66149 RPL32 antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-RPL32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66149

GTX66149 ICC/IF Image

ICC/IF analysis of L929 cells using GTX66149 RPL32 antibody.
Blue : DAPI
Dilution : 1:100

Anti-RPL32 antibody used in IHC (Paraffin sections) (IHC-P). GTX66149

GTX66149 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX66149 RPL32 antibody.
Dilution : 1:100

Anti-RPL32 antibody used in IHC (Paraffin sections) (IHC-P). GTX66149

GTX66149 IHC-P Image

IHC-P analysis of mouse spinal cord tissue using GTX66149 RPL32 antibody.
Dilution : 1:100

Anti-RPL32 antibody used in IHC (Paraffin sections) (IHC-P). GTX66149

GTX66149 IHC-P Image

IHC-P analysis of rat brain tissue using GTX66149 RPL32 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399