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RPL5 antibody

Anti-RPL5 antibody used in Western Blot (WB). GTX101821
Anti-RPL5 antibody used in Western Blot (WB). GTX101821
Anti-RPL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX101821
Anti-RPL5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101821
Anti-RPL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX101821
Anti-RPL5 antibody used in Western Blot (WB). GTX101821

Cat. No. GTX101821

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RPL5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Mouse brain , Rat2

Predict Reactivity

Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951757

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L5 , L5 , MSTP030 , PPP1R135 , uL18

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPL5 antibody used in Western Blot (WB). GTX101821

GTX101821 WB Image

RPL5 antibody detects RPL5 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
RPL5 antibody (GTX101821) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPL5 antibody used in Western Blot (WB). GTX101821

GTX101821 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPL5 antibody (GTX101821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX101821

GTX101821 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Gastric CA N87 xenograft, using RPL5(GTX101821) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101821

GTX101821 ICC/IF Image

RPL5 antibody detects RPL5 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPL5 protein stained by RPL5 antibody (GTX101821) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-RPL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX101821

GTX101821 IHC-P Image

RPL5 antibody detects RPL5 protein at cytoplasm on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
RPL5 antibody (GTX101821) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL5 antibody used in Western Blot (WB). GTX101821

GTX101821 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101821 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Rapone R et al. iScience 2023; 26 (8) : 107386 The cytoplasmic fraction of the histone lysine methyltransferase Setdb1 is essential for embryonic stem cells.
Naesens L et al. Sci Immunol 2022; 7 (77) : eabq4531 GTF3A mutations predispose to herpes simplex encephalitis by disrupting biogenesis of the host-derived RIG-I ligand RNA5SP141.
Haruka Matsumori et al. Life Sci Alliance 2022; 5 (7) Ribosomal protein L5 facilitates rDNA-bundled condensate and nucleolar assembly.
Application : WB
Reactivity : Human
Li Y et al. Oncogene 2020; (Epub) WDR74 modulates melanoma tumorigenesis and metastasis through the RPL5-MDM2-p53 pathway.
Application : WB
Reactivity : Human
Chiang JJ et al. Nat Immunol 2018; 19 (1) : 53-62 Viral unmasking of cellular 5S rRNA pseudogene transcripts induces RIG-I-mediated immunity.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169