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RPL5 antibody

Anti-RPL5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32848
Anti-RPL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX32848
Anti-RPL5 antibody used in Western Blot (WB). GTX32848
Anti-RPL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX32848

Cat No. GTX32848

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human RPL5 (NP_000960.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribosomal protein L5 , L5 , MSTP030 , PPP1R135 , uL18

Cellular Localization

Cytoplasm,Nucleolus

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18P family of ribosomal proteins and component of the 60S subunit. The encoded protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The encoded protein may also function to inhibit tumorigenesis through the activation of downstream tumor suppressors and the downregulation of oncoprotein expression. Mutations in this gene have been identified in patients with Diamond-Blackfan Anemia (DBA). This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. [provided by RefSeq, Mar 2017]

Database

Research Area

DATA IMAGES

Anti-RPL5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32848

GTX32848 ICC/IF Image

ICC/IF analysis of MCF-7 cell using GTX32848 RPL5 antibody.
Blue : DAPI

Anti-RPL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX32848

GTX32848 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX32848 RPL5 antibody.
Dilution : 1:200

Anti-RPL5 antibody used in Western Blot (WB). GTX32848

GTX32848 WB Image

WB analysis of various samples using GTX32848 RPL5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RPL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX32848

GTX32848 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX32848 RPL5 antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 329