GeneTex
United States (US)

RPL5 antibody

GTX32848_20180530_ICCIF_18121410_723.jpg
GTX32848_20180530_IHC-P_1_18121410_860.jpg
GTX32848_20180530_WB_18121410_996.jpg
GTX32848_20180530_IHC-P_18121410_779.jpg

Cat No. GTX32848

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

34 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human RPL5 (NP_000960.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ribosomal Protein L5,L5,Mstp030,Ppp1R135,Ul18,Rpl5

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in

Database

DATA IMAGES
GTX32848_20180530_ICCIF_18121410_723.jpg

GTX32848 ICC/IF Image

ICC/IF analysis of MCF-7 cell using GTX32848 RPL5 antibody.
Blue : DAPI

GTX32848_20180530_IHC-P_1_18121410_860.jpg

GTX32848 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX32848 RPL5 antibody.
Dilution : 1:200

GTX32848_20180530_WB_18121410_996.jpg

GTX32848 WB Image

WB analysis of various samples using GTX32848 RPL5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32848_20180530_IHC-P_18121410_779.jpg

GTX32848 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX32848 RPL5 antibody.
Dilution : 1:200

Package List Price ($)
$ 329