GeneTex
United States (US)

RPL7A antibody

GTX114727_40219_WB_M_18121410_401.jpg
GTX114727_40219_IHC_18121410_651.jpg
GTX114727_WB_18121410_110.jpg
GTX114727_40219_WB_R_18121410_521.jpg
GTX114727_40219_IFA_18121410_523.jpg

Cat No. GTX114727

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

A431 , Jurkat , Raji , Neuro2A , GL261 , C8D30 , PC-12 , Rat2

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL7A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ribosomal Protein L7A,L7A,Surf3,Trup,Rpl7A

Background

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene to form the chimeric oncogene trk-2h, which encodes an oncoprotein consisting of the N terminus of ribosomal protein L7a fused to the receptor tyrosine kinase domain of trk. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

DATA IMAGES
GTX114727_40219_WB_M_18121410_401.jpg

GTX114727 WB Image

Sample (30 ug of whole cell lysate)
A: Neuro2A
B: GL261
C: C8D30
12% SDS PAGE
GTX114727 diluted at 1:3000

GTX114727_40219_IHC_18121410_651.jpg

GTX114727 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using RPL7A(GTX114727) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114727_WB_18121410_110.jpg

GTX114727 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: JurKat
C: Raji
12% SDS PAGE
GTX114727 diluted at 1:1000

GTX114727_40219_WB_R_18121410_521.jpg

GTX114727 WB Image

RPL7A antibody detects RPL7A protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
RPL7A antibody (GTX114727) dilution: 1:1000

GTX114727_40219_IFA_18121410_523.jpg

GTX114727 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using RPL7A(GTX114727) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169