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RPL8 antibody

Anti-RPL8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117934
Anti-RPL8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117934
Anti-RPL8 antibody used in Western Blot (WB). GTX117934
Anti-RPL8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117934
Anti-RPL8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117934

Cat. No. GTX117934

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RPL8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Zebrafish, Drosophila, Bovine, Chicken, Japanese Medaka, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720771

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L8 , L8

Cellular Localization

Cytoplasm

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPL8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117934

GTX117934 IHC-P Image

RPL8 antibody detects RPL8 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
RPL8 antibody (GTX117934) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117934

GTX117934 IHC-P Image

RPL8 antibody detects RPL8 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
RPL8 antibody (GTX117934) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL8 antibody used in Western Blot (WB). GTX117934

GTX117934 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX117934 diluted at 1:1000

Anti-RPL8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117934

GTX117934 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using RPL8(GTX117934) antibody at 1:200 dilution.

Anti-RPL8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117934

GTX117934 IHC-P Image

RPL8 antibody detects RPL8 protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
RPL8 antibody (GTX117934) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Fittschen M et al. Neurogenetics 2015; 16 (3) : 181-92 Genetic ablation of ataxin-2 increases several global translation factors in their transcript abundance but decreases translation rate.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169