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RPL9 antibody

Anti-RPL9 antibody used in Western Blot (WB). GTX114728
Anti-RPL9 antibody used in IHC (Paraffin sections) (IHC-P). GTX114728
Anti-RPL9 antibody used in IHC (Paraffin sections) (IHC-P). GTX114728
Anti-RPL9 antibody used in IHC (Paraffin sections) (IHC-P). GTX114728
Anti-RPL9 antibody used in Western Blot (WB). GTX114728
Anti-RPL9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114728
Anti-RPL9 antibody used in IHC (Whole mount) (IHC-Wm). GTX114728
Anti-RPL9 antibody used in Western Blot (WB). GTX114728
Anti-RPL9 antibody used in Western Blot (WB). GTX114728

Cat. No. GTX114728

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all RPL9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Mouse brain , HeLa , HepG2 , HCT116

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619165

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L9 , L9 , NPC-A-16

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L6P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPL9 antibody used in Western Blot (WB). GTX114728

GTX114728 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114728 diluted at 1:1000

Anti-RPL9 antibody used in IHC (Paraffin sections) (IHC-P). GTX114728

GTX114728 IHC-P Image

RPL9 antibody detects RPL9 protein at retinal pigment epithelium in mouse eye by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
RPL9 antibody (GTX114728) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPL9 antibody used in IHC (Paraffin sections) (IHC-P). GTX114728

GTX114728 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RPL9 antibody(GTX114728) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL9 antibody used in IHC (Paraffin sections) (IHC-P). GTX114728

GTX114728 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using RPL9 antibody(GTX114728) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL9 antibody used in Western Blot (WB). GTX114728

GTX114728 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
C: HCT116
12% SDS PAGE
GTX114728 diluted at 1:1000

Anti-RPL9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114728

GTX114728 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using RPL9(GTX114728) antibody at 1:200 dilution.

Anti-RPL9 antibody used in IHC (Whole mount) (IHC-Wm). GTX114728

GTX114728 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using RPL9 antibody (GTX114728) at 1:200 dilution.

Anti-RPL9 antibody used in Western Blot (WB). GTX114728

GTX114728 WB Image

Sample (30 μg of whole cell lysate)
A: Zebrafish muscle
12% SDS PAGE
GTX114728 diluted at 1:1000

Anti-RPL9 antibody used in Western Blot (WB). GTX114728

GTX114728 WB Image

The data was published in the journal Cell Death Dis in 2016. PMID: 27253413

REFERENCE

Application Reference

Sbarrato T et al. Cell Death Dis 2016; 7 (6) : e2249 A ribosome-related signature in peripheral blood CLL B cells is linked to reduced survival following treatment.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169