GeneTex
United States (US)

RPL9 antibody

GTX114728_40219_IHC_R_18121410_121.jpg
GTX114728_40219_WB_M_brain_18121410_727.jpg
GTX114728_40219_IFA_18121410_718.jpg
GTX114728_40219_20161110_IHC-P_M_18121410_348.jpg
GTX114728_WB_18121410_428.jpg
GTX114728_40219_IHC_18121410_804.jpg

Cat No. GTX114728

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116 , mouse brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ribosomal Protein L9,L9,Npc-A-16,Rpl9

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L6P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX114728_40219_IHC_R_18121410_121.jpg

GTX114728 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using RPL9 antibody(GTX114728) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114728_40219_WB_M_brain_18121410_727.jpg

GTX114728 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114728 diluted at 1:1000

GTX114728_40219_IFA_18121410_718.jpg

GTX114728 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using RPL9(GTX114728) antibody at 1:200 dilution.

GTX114728_40219_20161110_IHC-P_M_18121410_348.jpg

GTX114728 IHC-P Image

RPL9 antibody detects RPL9 protein at retinal pigment epithelium in mouse eye by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
RPL9 antibody (GTX114728) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114728_WB_18121410_428.jpg

GTX114728 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
C: HCT116
12% SDS PAGE
GTX114728 diluted at 1:1000

GTX114728_40219_IHC_18121410_804.jpg

GTX114728 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RPL9 antibody(GTX114728) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169