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RPLP0 antibody, N-term

Anti-RPLP0 antibody, N-term used in Western Blot (WB). GTX47427
Anti-RPLP0 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47427

Cat No. GTX47427

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
50 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human RPLP0

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribosomal protein lateral stalk subunit P0 , L10E , LP0 , P0 , PRLP0 , RPP0

Cellular Localization

Nucleus,Cytoplasm

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RPLP0 antibody, N-term used in Western Blot (WB). GTX47427

GTX47427 WB Image

WB analysis of HepG2 cells using GTX47427 RPLP0 antibody at 0.2-1μg/ml.

Anti-RPLP0 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47427

GTX47427 IHC-P Image

IHC-P analysis of human bronchial epithelial tissue using GTX47427 RPLP0 antibody at 1:100. Left to right:DAPI, RPLP0, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf