Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPLP0 antibody (GTX114730) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RPLP0(GTX114730) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min