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RPLP2 antibody [HL2256]

Recombinant antibody
Anti-RPLP2 antibody [HL2256] used in Western Blot (WB). GTX638308
Anti-RPLP2 antibody [HL2256] used in Western Blot (WB). GTX638308
Anti-RPLP2 antibody [HL2256] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638308
Anti-RPLP2 antibody [HL2256] used in IHC (Paraffin sections) (IHC-P). GTX638308
Anti-RPLP2 antibody [HL2256] used in IHC (Paraffin sections) (IHC-P). GTX638308
Anti-RPLP2 antibody [HL2256] used in Western Blot (WB). GTX638308

Cat. No. GTX638308

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2256

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all RPLP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human RPLP2.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein lateral stalk subunit P2 , D11S2243E , LP2 , P2 , RPP2

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P1. The P2 protein can interact with P0 and P1 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RPLP2 antibody [HL2256] used in Western Blot (WB). GTX638308

GTX638308 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RPLP2 antibody [HL2256] (GTX638308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPLP2 antibody [HL2256] used in Western Blot (WB). GTX638308

GTX638308 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with RPLP2 antibody [HL2256] (GTX638308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-RPLP2 antibody [HL2256] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638308

GTX638308 ICC/IF Image

RPLP2 antibody [HL2256] detects RPLP2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: RPLP2 stained by RPLP2 antibody [HL2256] (GTX638308) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RPLP2 antibody [HL2256] used in IHC (Paraffin sections) (IHC-P). GTX638308

GTX638308 IHC-P Image

RPLP2 antibody [HL2256] detects RPLP2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
RPLP2 stained by RPLP2 antibody [HL2256] (GTX638308) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPLP2 antibody [HL2256] used in IHC (Paraffin sections) (IHC-P). GTX638308

GTX638308 IHC-P Image

RPLP2 antibody [HL2256] detects secreted RPLP2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
RPLP2 stained by RPLP2 antibody [HL2256] (GTX638308) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPLP2 antibody [HL2256] used in Western Blot (WB). GTX638308

GTX638308 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with RPLP2 antibody [HL2256] (GTX638308) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations