GeneTex
United States (US)

RPP40 antibody

GTX115483_41339_IFA_18121410_320.jpg
GTX115483_41339_20141128_WB_18121410_562.jpg

Cat No. GTX115483

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPP40. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RNASEP1 Antibody, ribonuclease P/MRP 40kDa subunit Antibody, bA428J1.3 Antibody

Cellular Localization

Nucleus , nucleolus

Background

Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.
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Database

DATA IMAGES
GTX115483_41339_IFA_18121410_320.jpg

GTX115483 ICC/IF Image

RPP40 antibody detects RPP40 protein at nucleus by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPP40 protein stained by RPP40 antibody (GTX115483) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX115483_41339_20141128_WB_18121410_562.jpg

GTX115483 WB Image

RPP40 antibody detects RPP40 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RPP40 antibody (GTX115483) diluted by 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169