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RPS10 antibody

Anti-RPS10 antibody used in IHC (Paraffin sections) (IHC-P). GTX101836
Anti-RPS10 antibody used in IHC (Paraffin sections) (IHC-P). GTX101836
Anti-RPS10 antibody used in Western Blot (WB). GTX101836
Anti-RPS10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101836
Anti-RPS10 antibody used in Western Blot (WB). GTX101836
Anti-RPS10 antibody used in IHC (Whole mount) (IHC-Wm). GTX101836
Anti-RPS10 antibody used in Western Blot (WB). GTX101836

Cat. No. GTX101836

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all RPS10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

Mouse brain , A431

Predict Reactivity

Rat, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPS10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951763

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S10 , DBA9 , S10

Cellular Localization

Cytoplasm

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPS10 antibody used in IHC (Paraffin sections) (IHC-P). GTX101836

GTX101836 IHC-P Image

RPS10 antibody detects RPS10 protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
RPS10 antibody (GTX101836) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPS10 antibody used in IHC (Paraffin sections) (IHC-P). GTX101836

GTX101836 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using RPS10(GTX101836) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPS10 antibody used in Western Blot (WB). GTX101836

GTX101836 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX101836 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPS10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101836

GTX101836 ICC/IF Image

RPS10 antibody detects RPS10 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPS10 protein stained by RPS10 antibody (GTX101836) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-RPS10 antibody used in Western Blot (WB). GTX101836

GTX101836 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX101836 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPS10 antibody used in IHC (Whole mount) (IHC-Wm). GTX101836

GTX101836 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using RPS10 antibody (GTX101836) at 1:200 dilution.

Anti-RPS10 antibody used in Western Blot (WB). GTX101836

GTX101836 WB Image

Sample (30 μg of whole cell lysate)
A: Zebrafish muscle
12% SDS PAGE
GTX101836 diluted at 1:500

REFERENCE

Application Reference

Ewan M Smith et al. Nucleic Acids Res 2021; 49 (1) : 458-478 The mTOR regulated RNA-binding protein LARP1 requires PABPC1 for guided mRNA interaction.
Application : WB
Reactivity : Human
Zhang X et al. J Virol 2021; (Epub) Neurovirulence of avian influenza virus is dependent on the interaction of viral NP protein with host factor FMRP in the murine brain.
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169