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RPS10 antibody

Anti-RPS10 antibody used in IHC (Paraffin sections) (IHC-P). GTX101836
Anti-RPS10 antibody used in IHC (Paraffin sections) (IHC-P). GTX101836
Anti-RPS10 antibody used in Western Blot (WB). GTX101836
Anti-RPS10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101836
Anti-RPS10 antibody used in Western Blot (WB). GTX101836

Cat No. GTX101836

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPS10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribosomal protein S10 , DBA9 , S10

Cellular Localization

Cytoplasm

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

DATA IMAGES

Anti-RPS10 antibody used in IHC (Paraffin sections) (IHC-P). GTX101836

GTX101836 IHC-P Image

RPS10 antibody detects RPS10 protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
RPS10 antibody (GTX101836) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPS10 antibody used in IHC (Paraffin sections) (IHC-P). GTX101836

GTX101836 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using RPS10(GTX101836) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPS10 antibody used in Western Blot (WB). GTX101836

GTX101836 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX101836 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPS10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101836

GTX101836 ICC/IF Image

RPS10 antibody detects RPS10 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPS10 protein stained by RPS10 antibody (GTX101836) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-RPS10 antibody used in Western Blot (WB). GTX101836

GTX101836 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX101836 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169