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RPS10 antibody

Anti-RPS10 antibody used in Immunoprecipitation (IP). GTX33475
Anti-RPS10 antibody used in Western Blot (WB). GTX33475
Anti-RPS10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33475
Anti-RPS10 antibody used in IHC (Paraffin sections) (IHC-P). GTX33475
Anti-RPS10 antibody used in IHC (Paraffin sections) (IHC-P). GTX33475

Cat. No. GTX33475

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all RPS10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:50
IHC-P 1:50 - 1:200
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human RPS10 (NP_001190174.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S10 , DBA9 , S10

Cellular Localization

Cytoplasm,Nucleolus

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternate splicing results in multiple transcript variants that encode the same protein. Naturally occurring read-through transcription occurs between this locus and the neighboring locus NUDT3 (nudix (nucleoside diphosphate linked moiety X)-type motif 3).[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-RPS10 antibody used in Immunoprecipitation (IP). GTX33475

GTX33475 IP Image

IP analysis of MCF-7 cell lysate using GTX33475 RPS10 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-RPS10 antibody used in Western Blot (WB). GTX33475

GTX33475 WB Image

WB analysis of various sample lysates using GTX33475 RPS10 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RPS10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33475

GTX33475 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX33475 RPS10 antibody.
Blue : DAPI

Anti-RPS10 antibody used in IHC (Paraffin sections) (IHC-P). GTX33475

GTX33475 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX33475 RPS10 antibody.
Dilution : 1:100

Anti-RPS10 antibody used in IHC (Paraffin sections) (IHC-P). GTX33475

GTX33475 IHC-P Image

IHC-P analysis of rat brain tissue using GTX33475 RPS10 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Lanzafame M et al. Nucleic Acids Res 2021; 49 (19) : 10911-10930 Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399