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RPS14 antibody

Anti-RPS14 antibody used in IHC (Paraffin sections) (IHC-P). GTX32849
Anti-RPS14 antibody used in Western Blot (WB). GTX32849
Anti-RPS14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32849

Cat No. GTX32849

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-151 of human RPS14 (NP_005608.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribosomal protein S14 , EMTB , S14

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-RPS14 antibody used in IHC (Paraffin sections) (IHC-P). GTX32849

GTX32849 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32849 RPS14 antibody.
Dilution : 1:100

Anti-RPS14 antibody used in Western Blot (WB). GTX32849

GTX32849 WB Image

WB analysis of various sample lysates using GTX32849 RPS14 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-RPS14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32849

GTX32849 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX32849 RPS14 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329