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RPS15 antibody

Anti-RPS15 antibody used in Western Blot (WB). GTX101839
Anti-RPS15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101839
Anti-RPS15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101839
Anti-RPS15 antibody used in Western Blot (WB). GTX101839
Anti-RPS15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101839
Anti-RPS15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101839

Cat No. GTX101839

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , MCF-7 , MDA-MB-231

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPS15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ribosomal protein S15 , RIG , S15

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm. This gene has been found to be activated in various tumors, such as insulinomas, esophageal cancers, and colon cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

DATA IMAGES
Anti-RPS15 antibody used in Western Blot (WB). GTX101839

GTX101839 WB Image

RPS15 antibody detects RPS15 protein by Western blot analysis.
A. 30 µg MCF-7
B. 30 µg MDA-MB-231
12 % SDS-PAGE
RPS15 antibody (GTX101839) dilution: 1:1000

Anti-RPS15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101839

GTX101839 IHC-P Image

RPS15 antibody detects RPS15 protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
RPS15 antibody (GTX101839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPS15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101839

GTX101839 IHC-P Image

RPS15 antibody detects RPS15 protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
RPS15 antibody (GTX101839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPS15 antibody used in Western Blot (WB). GTX101839

GTX101839 WB Image

RPS15 antibody detects RPS15 protein by Western blot analysis.
A. 30 µg U87-MG
B. 30 µg SK-N-SH
C. 30 µg IMR32
D. 30 µg SK-N-AS
12 % SDS-PAGE
RPS15 antibody (GTX101839) dilution: 1:1000

Anti-RPS15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101839

GTX101839 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using RPS15(GTX101839) antibody at 1:500 dilution.

Anti-RPS15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101839

GTX101839 IHC-P Image

RPS15 antibody detects RPS15 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
RPS15 antibody (GTX101839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169