Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RPS2 antibody [HL3338] (GTX641110) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS2 antibody [HL3338] (GTX641110) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.