GeneTex
United States (US)

RPS2 antibody [N2C3]

GTX114734_40233_WB_M_brain_18121410_343.jpg
GTX114734_40233_IFA_18121410_628.jpg
GTX114734_WB_18121410_461.jpg

Cat No. GTX114734

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Wm
Reactivity Human, Mouse, Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , mouse brain

Predict Reactivity

Rat, Drosophila, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ribosomal Protein S2,Llrep3,S2,Rps2

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S5P family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with mouse LLRep3. It is co-transcribed with the small nucleolar RNA gene U64, which is located in its third intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

DATA IMAGES
GTX114734_40233_WB_M_brain_18121410_343.jpg

GTX114734 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114734 diluted at 1:500

GTX114734_40233_IFA_18121410_628.jpg

GTX114734 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using RPS2(GTX114734) antibody at 1:200 dilution.

GTX114734_WB_18121410_461.jpg

GTX114734 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
12% SDS PAGE
GTX114734 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169