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RPS3 antibody

Anti-RPS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103964
Anti-RPS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103964
Anti-RPS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103964
Anti-RPS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103964
Anti-RPS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103964
Anti-RPS3 antibody used in Western Blot (WB). GTX103964

Cat. No. GTX103964

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RPS3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Predict Reactivity

Zebrafish, Drosophila, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPS3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037921

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S3 , S3

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103964

GTX103964 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RPS3(GTX103964) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-RPS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103964

GTX103964 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using RPS3(GTX103964) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103964

GTX103964 IHC-P Image

RPS3 antibody detects RPS3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
RPS3 stained by RPS3 antibody (GTX103964) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103964

GTX103964 IHC-P Image

RPS3 antibody detects RPS3 protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
RPS3 antibody (GTX103964) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103964

GTX103964 IHC-P Image

RPS3 antibody detects RPS3 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
RPS3 antibody (GTX103964) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPS3 antibody used in Western Blot (WB). GTX103964

GTX103964 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS3 antibody (GTX103964) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Imai H et al. Nucleic Acids Res 2023; 51 (14) : e76 Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification.
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169