Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RPS3(GTX103964) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS3 antibody (GTX103964) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

RPS3 antibody detects RPS3 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
RPS3 antibody (GTX103964) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RPS3 antibody detects RPS3 protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
RPS3 antibody (GTX103964) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RPS3 antibody detects RPS3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
RPS3 stained by RPS3 antibody (GTX103964) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using RPS3(GTX103964) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min