APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , PC-12 , Rat2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12
Product Note
Knockdown/Knockout validation was supported by references (PMID:26990993)
Predict Reactivity
Zebrafish, Bovine, Chicken, Pig, Japanese Medaka, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human RPS3A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951765
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ribosomal protein S3A , FTE1 , MFTL , S3A
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX105020 WB Image
RPS3A antibody detects RPS3A protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
12 % SDS-PAGE
RPS3A antibody (GTX105020) dilution: 1:1000
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GTX105020 IHC-P Image
RPS3A antibody detects RPS3A protein at cytosol on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
RPS3A antibody (GTX105020) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX105020 WB Image
Sample(30 ug whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
12% SDS PAGE
GTX105020 diluted at 1:1000
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GTX105020 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ribosomal protein S3a(GTX105020) antibody at 1:200 dilution.
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GTX105020 WB Image
RPS3A antibody detects RPS3A protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
RPS3A antibody (GTX105020) dilution: 1:1000
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REFERENCE
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