GeneTex
  • Country / Location Selection

United States (US)

RPS3A antibody

Anti-RPS3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105020
Anti-RPS3A antibody used in Western Blot (WB). GTX105020
Anti-RPS3A antibody used in Western Blot (WB). GTX105020
Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX105020
Anti-RPS3A antibody used in Western Blot (WB). GTX105020

Cat No. GTX105020

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Japanese Medaka, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPS3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ribosomal protein S3A , FTE1 , MFTL , S3A

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

DATA IMAGES
Anti-RPS3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105020

GTX105020 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ribosomal protein S3a(GTX105020) antibody at 1:200 dilution.

Anti-RPS3A antibody used in Western Blot (WB). GTX105020

GTX105020 WB Image

RPS3A antibody detects RPS3A protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
RPS3A antibody (GTX105020) dilution: 1:1000

Anti-RPS3A antibody used in Western Blot (WB). GTX105020

GTX105020 WB Image

Sample(30 ug whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
12% SDS PAGE
GTX105020 diluted at 1:1000

Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX105020

GTX105020 IHC-P Image

RPS3A antibody detects RPS3A protein at cytosol on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
RPS3A antibody (GTX105020) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPS3A antibody used in Western Blot (WB). GTX105020

GTX105020 WB Image

RPS3A antibody detects RPS3A protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
12 % SDS-PAGE
RPS3A antibody (GTX105020) dilution: 1:1000

REFERENCE
REVIEW
There are currently no reviews for RPS3A antibody (GTX105020). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169