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RPS3A antibody

Anti-RPS3A antibody used in Western Blot (WB). GTX111935
Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX111935
Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX111935
Anti-RPS3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111935
Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX111935
Anti-RPS3A antibody used in Western Blot (WB). GTX111935

Cat. No. GTX111935

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all RPS3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

A431 , Raji

Predict Reactivity

Bovine, Chicken, Pig, Japanese Medaka, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human RPS3A Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176177

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S3A , FTE1 , MFTL , S3A

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPS3A antibody used in Western Blot (WB). GTX111935

GTX111935 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: Raji
12% SDS PAGE
GTX111935 diluted at 1:1000

Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX111935

GTX111935 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RPS3A antibody(GTX111935) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX111935

GTX111935 IHC-P Image

RPS3A antibody detects RPS3A protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
RPS3A antibody (GTX111935) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPS3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111935

GTX111935 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPS3A(GTX111935) antibody at 1:500 dilution.

Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX111935

GTX111935 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using RPS3A antibody (GTX111935) at 1:300 dilution.

Anti-RPS3A antibody used in Western Blot (WB). GTX111935

GTX111935 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
12% SDS PAGE
GTX111935 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169