GeneTex
United States (US)

RPS3A antibody

Anti-RPS3A antibody used in Immunoprecipitation (IP). GTX33478
Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX33478
Anti-RPS3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33478
Anti-RPS3A antibody used in Western Blot (WB). GTX33478
Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX33478
Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX33478

Cat No. GTX33478

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human RPS3A (NP_000997.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ribosomal Protein S3A,Fte1,Mftl,S3A,Rps3A

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]

Database

DATA IMAGES
Anti-RPS3A antibody used in Immunoprecipitation (IP). GTX33478

GTX33478 IP Image

IP analysis of MCF7 cell lysate using GTX33478 RPS3A antibody.
IP reaction : 3μg antibody / 200μg cell lysate
Dilution : 1:500

Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX33478

GTX33478 IHC-P Image

IHC-P analysis of rat brain tissue using GTX33478 RPS3A antibody.
Dilution : 1:100

Anti-RPS3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33478

GTX33478 ICC/IF Image

ICC/IF analysis of MCF-7 cell using GTX33478 RPS3A antibody.
Blue : DAPI

Anti-RPS3A antibody used in Western Blot (WB). GTX33478

GTX33478 WB Image

WB analysis of various samples using GTX33478 RPS3A antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX33478

GTX33478 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX33478 RPS3A antibody.
Dilution : 1:100

Anti-RPS3A antibody used in IHC (Paraffin sections) (IHC-P). GTX33478

GTX33478 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX33478 RPS3A antibody.
Dilution : 1:100

Package List Price ($)
$ 329