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RPS6 (phospho Ser235/236) antibody

Orthogonal
Anti-RPS6 (phospho Ser235/236) antibody used in Western Blot (WB). GTX12864
Anti-RPS6 (phospho Ser235/236) antibody used in Western Blot (WB). GTX12864

Cat. No. GTX12864

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all RPS6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Product Note

This antibody does not cross react with RPS6 phosphorylated on serines 244 and 247.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human RPS6 that contains serines 235 and 236. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_367025

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 , S6

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RPS6 (phospho Ser235/236) antibody used in Western Blot (WB). GTX12864

GTX12864 WB Image

Orthogonal

WB (peptide competition) analysis of HeLa cells treated with anisomycin (Lane 2-5) using GTX12864 RPS6 (phospho Ser235/236) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Anti-RPS6 (phospho Ser235/236) antibody used in Western Blot (WB). GTX12864

GTX12864 WB Image

Orthogonal

WB analysis of whole cell extracts (20 μg lysate) of HeLa (Lane 1), treated for 20 minutes with 200 nM of PMA (Lane 2), A549 (lane 3) serum starved A549 (lane 4) and serum starved for overnight followed by Serum Released (lane 5) using GTX12864 RPS6 (phospho Ser235/236) antibody.
Dilution : 1:500

REFERENCE

Application Reference

Goodson WH 3rd et al. Carcinogenesis 2011; 32 (11) : 1724-33 Activation of the mTOR pathway by low levels of xenoestrogens in breast epithelial cells from high-risk women.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399