WB analysis of whole cell extracts (20 μg lysate) of HeLa (Lane 1), treated for 20 minutes with 200 nM of PMA (Lane 2), A549 (lane 3) serum starved A549 (lane 4) and serum starved for overnight followed by Serum Released (lane 5) using GTX12864 RPS6 (phospho Ser235/236) antibody.
Dilution : 1:500

WB (peptide competition) analysis of HeLa cells treated with anisomycin (Lane 2-5) using GTX12864 RPS6 (phospho Ser235/236) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.